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welcome to the applied biosystems tech talk video series where we break down real-time pcr to make it easier for you if youamp;#39;ve ever been in the market for a real-time pcr master mix youamp;#39;ve likely heard of rock style itamp;#39;s so ubiquitous that maybe youamp;#39;ve never stopped to think about why so letamp;#39;s talk about it rockstye is an inert fluorescent molecule that can be detected by a real-time pcr instrument when itamp;#39;s present in reactions but unlike fam vic and applied biosystems cybergreen master mix reporter dies rox fluorescence doesnamp;#39;t change as a direct result of pcr amplification instead rox just sits there now you may be asking whatamp;#39;s the point well in any real-time pcr system there are several potential sources of well-to-well variability in reporter molecule excitation and signal detection these include bubbles in wells and even differences in the degree of condensation rox normalizes for these and other sources of
