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so welcome to another practical demonstration of staining techniques this time weamp;#39;ll be comparing two types of elastin stain or scene and the ha schema toxin on technique so weamp;#39;ve already prepared some sections of esophagus and the sections to be staying with the Hoff so youamp;#39;re sitting here in the water bath just adjust the water level there a little bit the all scene however the slides only need to be taken down as far as 70% alcohol because the or seen stain is effectively in a fairly alcoholic solution so thatamp;#39;s as far as we need to go where the very taken them all all the way down to tap water okay so now itamp;#39;s useful to compare these two staining techniques side by side just to better illustrate the similarities and differences beginning with some similarities youamp;#39;ll notice weamp;#39;ve got four petri dishes here and thatamp;#39;s because itamp;#39;s advantageous to stain all of these slides upside down just to prevent any