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Wright-Giemsa Stains, also known as Romanowsky Stains, are stains used in hematology and cytological studies to differentiate cells in microscopic examinations of peripheral blood bone marrow specimens. These stains can also be applied to detect the presence of parasites in the blood, such as the Plasmodium parasite that causes malaria, when rapid results are needed. There are a variety of Romanowsky stain types that apply the same principle. They include Giemsa stain Wright and Wright-Giemsa stain, May-Grunwald stain, and Leishman stain. This complex of stains was named after Dmitri Leonidovich Romanowsky, a Russian Physician who first identified the importance of using blood samples to diagnose hematological disease in 1891. The principle of Romanowsky stains is that they are made up of oxidized methylene blue dyes and Eosin Y. The Azure dies are basic and bind to acid nuclei forming a blue to purple color. The acid dye, Eosin, binds to alkaline cytoplasmic components
