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foreign manual modified Papua Nicolaus staining solution demonstration preparation before operation slides semen sample pipette modified Papua Nicolaus staining solution staining tray microscope wash bottle timer 95 ethanol Step 1 prepare a clean slide for later use mix the semen sample well use a pipette to take the semen sample Place 5 to 10 microliter of semen on a clean slide evenly spread the semen by crawling with another clean slide let the smear air dry Step 2 add 95 ethanol on the semen smear wait for three minutes to let the semen smear get fixed rinse the Siemens mirror with purified water to remove ethanol step 3 take the solution of the carried modified pop nicolao staining kit and add on the Siemens mirror covering it as much as possible wait for three minutes to let the staining happen rinse the semen smear with purified water to remove the solution a step 4 take the solution B of the carried modified Papua nicolao staining kit and add on the Siemens mirror cover
