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hello everyone this is Marlene and dr. Hinton we are now going to start the missile staining procedure weamp;#39;re gonna be following the protocol so this is what your slides that you would have mounted would look like you would have mounted your sections in order from rostral to caudal and so we would allow this to dry for a few days before starting the missile staining protocol so the first step was on the protocol to to mount on sub slides now one of the most important things is weamp;#39;re not allowed to let our sections dry between subsequent solution changes that weamp;#39;re going to be doing right now for the missile staining so the first step is to dehydrate and deliver dyes so we start this process with 95 percent alcohol the protocol says 3 minutes but given that we are in special circumstances right now we are truncating this down to a minute so so more than go ahead letamp;#39;s start off with the 95 percent alcohol weamp;#39;ll just leave the slides in there for ab
