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we have completed tissue staining now we will mount a cover slip on the tissue slide we have the de-paraphanized h e stain slides we have previously prepared mounting medium and coverslips place the slides on a flat clean non-absorbent work surface some residual droplets May remain using a whiteboard pipette tip add 100 microliters mounting medium to uniformly cover all tissue sections on the slide apply the coverslip at an angle on one end of the slide slowly lower the cover slip without introducing bubbles allow the mounting medium to spread and settle if needed remove any large excess of mounting medium by carefully wicking away from the edge of the coverslip with a laboratory wide be careful not to move the cover slip and disturb the tissue after completing h d standing in cover slip mounting slides can be stored laying flat in a container at 4 degrees Celsius in the dark for less than 24 hours do not use citaseal or nail polish for securing the cover slip please refer to t