Complete columns certificate easily

Aug 6th, 2022
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Most of the HPLC columns currently available are based on silica gel. Silica gel is a solid, amorphous silicon dioxide used for HPLC applications in the form of small particles of defined size.
Generally, silica gel is filled in the high-performance liquid chromatography columns because of its particle size and porosity that helps in separation of components and silica gel is also an inert material that does not react with mobile phases.
Silica-based HPLC columns. Most of the HPLC columns currently available are based on silica gel. Silica gel is a solid, amorphous silicon dioxide used for HPLC applications in the form of small particles of defined size.
Size exclusion HPLC columns are used primarily for the separation of proteins and carbohydrates. Other types of HPLC columns include affinity, ion exclusion, and displacement chromatography columns; a chiral HPLC column can be used to resolve racemic mixtures.
Silica Gel for Column Chromatography is used in organic chemistry for purification and isolation of synthetic compounds Silica Gels provide an ideal stationary phase for optimum and reproducible purification.
C18 is octyldecylsilane and contains 18 carbons bound to the silica. So they have more carbons and a longer carbon chain than C8 (8 carbons) or C4 (4 carbons). Because of the extra carbons, C18 has a larger surface area that the mobile phase has to travel across.

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