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welcome to the applied biosystems tech talk video series where we break down real-time pcr to make it easier for you relative gene expression analysis is one of the most common real-time pcr applications with one of the most common real-time pcr questions since every cdna is run with both the gene of interest and an internal control do i have to be sure the concentration of each cdna is the same letamp;#39;s talk about it whenever we do a gene expression experiment we need at least two gene-specific assays one for our gene of interest and one for an endogenous control gene also referred to as a normalizer or housekeeping gene different terms same idea so what does the endogenous control do for us well essentially its number one job is to normalize our data to account for differences in the amount of template added hereamp;#39;s what i mean say iamp;#39;m comparing expression of my gene of interest using two samples one untreated and the other from a treated cell line when i
