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you a problem that can occur with agar plates is the presence of black or dark particles that are caused by burning culture medium here is a low slicing deoxy coli taker or XRD for the detection of salmonella if prepared correctly the agar is a clear red gel you can see here that there are black particles throughout the egg oh this is especially a problem for this agar as the target organism Salmonella growers his black colonies on the agar thus burnt particles could be misinterpreted for a pathogen this is an extreme level of burning and users may normally only see only a couple of charred particles one way this can occur is the improper dissolution of powder here I have added the powder to the water as you can see it sits on top of the water before slowly dissolving if heat is applied now before all powder is dissolved parts the powder may burn and formed the precipitate you saw earlier to prevent this from happening powder should be fully solubilized before heating you
