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how to collect sputum samples. Sputum samples are used to diagnose active tuberculosis also known as TB and to monitor the effectiveness of TB treatment. Youamp;#39;ve already been given up to three plastic bottles to collect sputum samples in this step-by-step video weamp;#39;ll show you how to properly collect those samples itamp;#39;s important to collect the sputum sample right after you wake up in the morning because thick sputum collects in the lungs overnight. Do not eat or drink, brush your teeth, smoke or use mouthwash before you collect your sputum. Before starting wash your hands with soap and water for 15 to 20 seconds Rinse well and use a hand towel to pat dry. on the bottle write your name as well as the date and time the sputum sample was collected remember to use a different bottle each day, next open the plastic bottle but be careful not to touch the inside of the bottle or the cap. now breathe in deeply two to three times and then cough deeply from the chest to bri