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before we begin the clean flex targeted library preparation protocol itamp;#39;s important to have all the instruments supplies and reagents ready to ensure successful completion of the protocol in this video we will show you how to get ready to construct your clean flex libraries each clean Flex NGS panel contains a clean flex targeted library kit and panel specific clean collects multiplex PCR primers clean flex index PCR primers and clean Mac magnetic beads are purchased separately to complete the workflow from input DNA sequencing ready NGS libraries you should have the following equipment and supplies to help you complete the protocol single or multi-channel pipettes low retention pipette tips a bench top mini centrifuge a thermal cycler of vortex PCR tubes PCR tube racks 100 percent molecular biology grade ethanol nucleus free water Coll tube rack hook - pedometer and dsDNA high sensitivity as a kit and an Agilent bioanalyzer and highs in the tivity DNA kit first