Clear up motif in PAP

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Aug 6th, 2022
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How to clear up motif in PAP

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to them sometimes we cannot do justice in terms of a final diagnosis so here what the faculty has tried to do is to you know give you a little uh Journey Down pychology samples of how whether uh routine non-routine basic conventional liquid based ancillary what all is possible see it may not be available at your Center but at least you can guide the clinician that this is it and you can get this done and the situations in which where there is a difficulty of how to get out of a situation and you know suggest something weamp;#39;ll be giving such ideas also so I hope you enjoy this so I hope you enjoy this Iamp;#39;m Dr kti uh I had the enop paath division at Metropolis after experience in hinduja prin alikhan gangaram and various such places basically trained in the armed forces and um I have uh Kane Madam is going to come and uh uh take the thing and introduce the subject and uh weamp;#39;ll just start the journey from here hello good morning everybody yeah Iamp;#39;m really surpr

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Wash slides in deionized or distilled water for 5 min at room temperature. Destain slides in 0.5% HCl in 70% ethanol for 5-15 min at room temperature. Wash slides in deionized or distilled water for 5 min at room temperature. Air dry sections.
Common tumor markers for cervical cancer include: cancer antigen 125 (CA 125) carcinoembryonic antigen (CEA) cancer antigen 19-9 (CA 19-9)
Normal Pap test results: No abnormal cervical cells were found. A normal test result may also be called a negative test result or negative for intraepithelial lesion (area of abnormal growth) or malignancy.
Typically, your doctor will use the Loop Electrosurgical Excision Procedure (LEEP), or perform a cold knife cone biopsy as a minor surgical procedure. Be sure to always bring up any questions you may about you Pap test with your gynecologist.
Ethyl alcohol is the fixative specifically recommended for cytological preparations. Fixation coarsens the cell structures and sharpens nuclear chromatin pattern and its details.
Pap Staining involves five dyes in three solutions, a nuclear stain and two counter stains: Nuclear Stain: Harris Haematoxylin which is used to stain cell nuclei. Counter Stain OG6: Orange G dye is used to stain keratin. Its original role was to stain the small cells of keratinizing squamous cells present in sputum.
Saline solution may be used for moistening. The lubricant does not dissolve during processing of the slide. It often stains dark purple and may hide numerous cells. Before scraping, the wooden spatula should be dipped in saline to moisten it and prevent too rapid dehydration of the scraped cells.
The pap stain has 3 solutions having 6 dyes. Solution 1 has Hematoxylin stain, a basic nuclear stain. Solution 2 has OG-6, an acidic cytoplasmic stain, has orange G with phosphotungstic acid (6 denotes the used concentration of phosphotungstic acid).

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