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has come from well theyamp;#39;re not just manifested by sequencers instead theyamp;#39;re produced by recording patterns of intensity over time typically fluorescent intensity as a function of time now what Iamp;#39;m showing you here is an example from a Sanger sequencer kind of the old type of sequencer today this is produced by a capillary electrophoresis fluorescence and measurement of the complementary strands being produced being polymerized for a template strand if you were to look at region the region around nucleotide 200 in this electropherogram you would see patterns of intensity that are nice and regularly spaced and you see that what letter should be called for each position is pretty unambiguous we have a few places where the black fluorescence is at the peak those represent G we have places where the red peak is highest thatamp;#39;s a T green for a and blue for G all fairly straightforward we do however see that when we get to very high parts of the sequence when w
