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welcome back to the high seat expert video tip series in this video we will continue providing tips for the high seat fluidic system something we began in part four of this series also if youamp;#39;ve not already done so be sure to check out parts 1 2 amp;amp; 3 of this video series part 1 provided tips related to sequencing primers part 2 discussed tips for the instrument computer part 3 covered best practices for the thermal system mechanical system and instrument maintenance a useful skill to have when operating at high seek is how to determine where a fluidic line clog or blockage is located clocks can be identified by two characteristics slow flow or no flow at all through the flow cell lane or back flow as seen here backflow can result due to the binary splitting of reagent lines Laneamp;#39;s 1 amp;amp; 2 3 amp;amp; 4 5 amp;amp; 6 amp;amp; 7 amp;amp; 8 share a direct connection just before where the reagent enters the flow cell a blockage in one of the lane pair