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the method is complete now we will perform instrument cleanup after the protograph assay method is complete the ics presents two options unload carriers and run mpe flush this is the recommended option the mpe flush will take approximately 10 minutes unload carriers only if this option is selected the mpe flush method can be accessed from the maintenance menu from the main screen once an option is selected the carriers will automatically move from the work deck into the auto load tray area immediately remove the peptide collection plate which contains the clean peptides seal the plate with an adhesive mat and spin down the plate the resulting sample plate layout is shown in the user guide set aside the plate at room temperature start peptide quantification within one hour next we will remove and dispose of the labware from the carriers open the front protective cover remove all plastic labware from the tube plate and reservoir carriers remove the reservoirs and store the black