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Before you get started, add 4 volumes of ethanol to one volume of DNA Wash Buffer according to the instructions on the bottles label. All centrifugation steps should be carried out at 16,000 x g (~13,000 RPM) BINDING We recommend starting with a DNA sample size of 20-100 l. Add the recommended amount of DNA Cleanup Binding Buffer to your sample. The ratio of DNA Cleanup Binding Buffer volume to add into your sample volume is dependent on the size and type of DNA in your sample. Refer to the table on the protocol card to determine this amount. If you are working with DNA sample volumes smaller than 20 l, you will need to first increase these volumes to at least 20 l by adding TE buffer. If your sample is greater that 800 l after being diluted with binding buffer, you will need to load the column with a portion of it, spin, and then load the rest, as the column reservoir may not be able to contain all of it at once. Mix well by pipetting up and down or flicking the tube. Load the s