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A lot of you have been asking me whether to do a size selection or a simple cleanup for the NEBNext Ultra DNA or RNA workflows. The first consideration would be whether you need a precise insert range. If you need a precise insert range, you would have to do a size selection. The other factor is, how much starting material you have. If your starting material is low and you are doing a size selection you could potentially reduce the complexity of your library. So, if your input amount is low, just do a cleanup. It is always important to remove adaptor dimer. If you have adaptor dimer after the PCR cleanup, you can do another 0.9x SPRI bead ratio cleanup. If you have any further questions, you can always docHub us at info@neb.com.
