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[Music] so here we have two slides that have been taken down to water for the purpose of staining with the PAS method so the P of course stands for periodic acid thats an oxidizing agent and well be applying that to a section of liver and to a section of kidney so quite different in terms of what we expect to see just give these a bit of a rinse with deionized water its less critical at this point but since there are chemical reactions taking place it doesnt hurt to have something fairly clean on the on the slide so as flick that off and then take our periodic acid now the length of time that we need it does differ depending upon what were staining for something like liver where we may have glycogen about 10 minutes is sufficient and for kidney were mainly looking at basement membranes so wed need to give that a bit longer up to about 20 minutes but just for the purpose I think of this demonstration for both this one and the other one I think well give that about 15 minutes so