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[Applause] [Music] to begin your tem session check the vacuum and the ht lamps are lit log on using your client name and client number click on the techno user interface icon and wait for the server to start click on the vacuum overview and check the vacuum status once the vacuum is stable fill the anti-contaminator with liquid nitrogen use appropriate personal protective equipment [Music] to remove the sample holder pull out the holder till it docHubes a stop rotate it clockwise about 145 degrees place your finger against the goniometer and push gently to break the vacuum and then remove the holder [Music] use the guide pin to open the clamp [Music] place the sample in position [Music] close the clamp rotate the sample 90 degrees gently tap the rod to check that the sample doesnt fall out using the light microscope check the o-ring on the sample rod check that its clean the opto blade and the guide pin need to line up with the goniometer carefully insert the sample holder into the go