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Hello and welcome back to the channel today in our continuing FlowJo adventures were going to look at some basic steps to perform clean up on your flow cytometry data. So I have just a basic experiment here two samples stained and unstained blood on a 12 color panel. Again Ive previously analyzed these but just to work through my data cleanup process. So the first thing I would do would be to open the data file and just do a basic forward scatter side scatter gate so I can get rid of the debris, the clumps, and the really obvious stuff that I dont want to analyze. So something along those lines. Again Im not fine-tuning into the exact population I want to look at- just a broad gate so I can get rid of the debris, clumps, things I dont need. From there we could go the old school way and do something like a fluorescence versus time. So if we look at our fluorescence versus for one of our parameters- say CD3- you can see we had fairly steady fluidics un