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Size selection enriches for molecules that were sheared to the desired size and have an adaptor ligated to each end. Size selection is accomplished using magnetic beads. There are two rounds of selection: the first removes DNA fragments larger than the desired size, and the second removes DNA fragments smaller than the desired size. This is accomplished using specific ratios of the bead solution to total volume, and the volume of beads required varies depending on the desired fragment size. Magnetic beads should be used at room temperature and they should be vortexed before use. For the first round of size selection add vortexed beads to the reaction and mix well by pipetting up and down at least 10 times. Incubate for five minutes at room temperature, then place the reaction into the magnetic field. After approximately five minutes the beads should have separated from the supernatant. Carefully remove the supernatant and avoid disturbing the pellet. Vertical bar magnets pull the bead
