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hey guys youre watching Python tutorial videos on my youtube channel Python for microscopy so in todays tutorial Im gonna talk about estimating image quality without using any reference images in the previous video I talked about using a reference to quantify certain metrics like peak signal-to-noise ratio or mean squared error between the two images that you have now here we are talking about not using any reference okay how do we do that by quantifying sharpness and Im going to talk about how we do that in a second but Ive already covered a topic called no reference image quality assessment using a technique called breast BR is qu e go ahead and try that in addition to the one Im going to show you to see which one works great for your images there is no single magic formula that works on all the images thats why Im trying to cover as much as I can based on the papers that got published out there preferably with code thats already out there so we dont have to do a bunch of