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reasons for cleaning and disinfection the predominant reason for cleaning and disinfection of endoscopes is to prevent the transmission of disease this is achieved by observing standard precautions this includes hand-washing wearing personal protective clothing and utilizing the appropriate method of reprocessing for flexible endoscopes infectious agents that can be transmitted by in adequately cleaned endoscopes and accessories include bacteria such as Helicobacter Pseudomonas Salmonella viruses including hepatitis B virus and hepatitis C virus and others such as prions the most important rule of any effective reprocessing is clean it clean it clean it the most important step in endoscope reprocessing is thorough manual cleaning prior to disinfection or sterilization endoscopes that have not been adequately cleaned cannot be properly disinfected or sterilized even with prolonged exposure times in order for manual cleaning to be effective it must be performed by a person familiar with