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the cleaning of reusable dental instruments prior to heat sterilization is a crucial yet sometimes misunderstood infection control procedure if biological contamination is not removed by proper cleaning the remaining bioburden can interfere with the lethality of the sterilization cycle cleaning should be performed as soon as possible in a designated area using the appropriate personal protective equipment be sure to clean instruments according to the manufacturers instructions for use paying attention to detergent type and dilution water quality and temperature be sure to use approved cleaning solutions as household products not intended for use with instruments can cause damage and/or limit cleaning effectiveness if cleaning cannot be performed immediately pre-soaking the instruments in a neutral pH detergent or enzymatic cleaner will prevent drying of the bioburden and dental materials to make the cleaning easier and less time-consuming there are manual and mechanical methods of cl