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Summarized text: In this video tutorial, parameters such as column size, type of chromatography resin, packing volume, and flow rate are essential for operation. Follow experimental requirements and resin instructions when estimating the amount of slurry needed based on the final column volume. Resins are typically delivered in a 75% slurry concentration in storage solution. To calculate the necessary slurry volume for packing a column, use a compression factor (often 1.15) and ensure thorough washing of the resin before packing. Use a fritted glass funnel for efficient packing.