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this videos about breakthrough curves in chromatography columns and require basic knowledge on order breakthrough curve means for a chromatography column what the breakpoint to be is and what T star tells you about the adsorption capacity of the column I will in this video explain two things related to batch wise adsorption in column firstly why T star is scalable with column length during up scaling while TB the breakpoint is not and secondly why a favorable isotherm is required to achieve constant pattern in batch vise : adsorption we start with a column filled with water or some other liquid thus that was not adsorbed to the adsorbent the water is blue in this animation and the red is the substance being adsorbed the darker the red the higher their concentration in the water in this second animation I have instead illustrated the front as a concentration curve that moves along the column the two animations are two ways to illustrate exactly the same thing the breakthrough curve is a