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hi everyone this is Annie and Iamp;#39;m going to be showing you the method of how to complete a differential scanning flry or DSF melt curve so this video is going to cover some background the methods of the experiment and then finally where to find the stuff in our lab so differential scanning flry or DSF M curves are used to determine the melting temperature of a protein or a protein Li complex and the way it works is you combine a protein or protein Lian complex with a Dy that when the Dy binds to a hydrophobic region it fluores and so as you increase temperature and your protein unfolds more and more of the internal hydrophobic regions are exposed the fluorescent die binds and you measure the resulting fluoresence it results in a graph that looks like this where you start at low fluoresence increasing the temperature moving to the right and the floressence increases as the protein melts the the melting point is determined at the inflection point and so thatamp;#39;s about when 5
