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[Music] welcome back to bio techniques on catalyst University my name is Kevin toke off please make sure to like this video and subscribe to the channel for future videos and notifications in this video were gonna discuss the principles of primer design for PCR polymerase chain reactions so how do you get the specific forward and reverse primers given a DNA sequence such as the one shown here and this DNA sequence is the sequence for the gene or one of the actin proteins okay and we can tell that its a gene first of all because it has Ts in it so its its from the DNA has thymine also the first three letter are three nucleotide triplet here is ATG this is actually the triplet code for methionine if we imagine the mRNA from this would be Aug thats our start codon so this is methionine down here on the three prime end we have the nucleotide triplet TGA as a codon and mRNA to be UGA which is a stop codon so this is the entire gene sequence for active how do we get the primers from th