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well once weamp;#39;ve transferred the proteins from the gel to the membrane we have a choice of either using the benchpro 4100 Western processing system to do the rest of the western or to get results even sooner we could use the iblot Western detection system so letamp;#39;s begin by unboxing the iblot Western detection kit the kit comes with two boxes here I have the stacks for performing The Western and then I have a box containing the reagents so letamp;#39;s see what we get in the reagent box so we have a manual we have a couple of incubation trays and then we have some um antibody diluent solution we have three bottles of these which you will use not just for diluting the antibody but also as a blocking solution we have some uh wash Solutions couple bottles of those and then we have a substrate this is a chemoluminescent kit so we get a chemoluminescent substrate we have two tubes of secondary antibody we have a antibody diluent additive and then we have the substrate enhance