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welcome back today weamp;#39;ll be talking about the blotting tests and I try to cover everything that youamp;#39;ll need to know for you step 1 exam before we jump into the mechanism youamp;#39;ll have to know that we use the blotting test to detect the presence of certain segments of DNA or detect the presence of certain gene expression or the presence of a specific protein and this is how this is done so weamp;#39;ll start off on the sample of DNA that may or may not contain the certain segments of DNA that you are looking for weamp;#39;ll use cleaving enzymes to take off one strand of the DNA and weamp;#39;ll add a radio-labelled primer that is specific to that DNA that we want for example if weamp;#39;re looking for HIV DNA weamp;#39;ll add label primers to that HIV DNA if that sample of DNA did in fact contain some HIV DNA this primer very specific for that HIV DNA will link to this segment and then we will be able to detect it now the process of detecting a specific prot
