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good morning everyone this is professor Robinson in this lecture today Iamp;#39;m going to discuss about the Western blotting one of the very commonly used method in biochemistry to detect the presence of a specific protein in a given sample the method of Western blotting idiot Elizaamp;#39;s antibody to detect the presence of a specific protein in a given sample and this sample can be sterilized it or be blood plasma or any other sample so what happens is that we have a good example and in this sample there are four different types of proteins present letamp;#39;s say protein a B C and D and we want to detect this protein specifically then we will use this method of Western blotting to detect this protein a specifically so like I said before the method of Western blotting utilizes antibody and there are two different types of antibody that are used one is primary antibody and the other is secondary antibody our prime the antibody is specific to protein a if we want to detect this p