Blot type in LOG

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Aug 6th, 2022
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How to blot type in LOG

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In molecular biology and genetics, a blot is a method of transferring large biomolecules (proteins, DNA or RNA) onto a carrier, such as a membrane composed of nitrocellulose, polyvinylidene fluoride or nylon.
Blotting is a technique by which a macromolecule such as DNA, RNA, or protein is resolved in a gel matrix, transferred to a solid support, and detected with a specific probe. These powerful techniques allow the researcher to identify and characterize specific molecules in a complex mixture of related molecules.
Southern blot detects specific DNA sequences, Northern blot detects particular RNA sequences, and Western blot detects specific proteins. All these techniques are complementary. Furthermore, it is worth saying that, even though the methods used are very similar, the processes all differ a bit from the others.
However, the main difference that has to be noted is the molecule that each technique is meant to identify. Southern blot detects specific DNA sequences, Northern blot detects particular RNA sequences, and Western blot detects specific proteins. All these techniques are complementary.
Three main types of blots are used regularly in laboratories. Western blot for proteins, Northern blot for RNA, and though not very common, Southern blot for DNA. There can be slight variations to these blots, each for a specific experimental purpose.
Major types of blotting techniques include Southern blotting (for DNA), Northern blotting (for RNA), Western blotting (for proteins), Southwestern blotting (for DNA-protein interactions), and Eastern blotting (for post-translational modifications of proteins).
Table of Blotting Types Blotting TypeTarget MoleculeDetection Method Southern Blotting DNA Labeled DNA/RNA probes Northern Blotting RNA Labeled DNA/RNA probes Western Blotting Proteins Primary/secondary antibodies Eastern Blotting Modified proteins Specific probes/antibodies6 more rows Feb 15, 2024

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