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hey guys cook by chemstro 86-year letamp;#39;s talk about northern blot northern blot is a molecular biology technique used for the detection of specific RNA and this technique iron is first isolated and separated by agarose gel electrophoresis the RNA separated in the gel is transferred to the nylon membrane for this purpose the nylon membrane is placed on the gel and fill the papers are placed on the top the cellist and transferred in the buffer due to capillary action the filter paper absorbs the buffer and the RNA present in the gel by instan alone membrane the interaction between RNA and the membrane is favored by charge interaction the nylon membrane has a positive charge while RNA has a negative charge because of this reason RNA easily binds the membrane once binding occurs the RNA is immobilized on the membrane by treating the membrane with UV light or heat once RNA is immobilized this treated with labelled probe the probe can either be single-stranded DNA or RNA labeled with
