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hello my name is alyssa antone hello and i am dr resters in spots we are using western blotting to learn how drugs get into the brain and what determines how they get there western blotting will measure relative expression of p glycoprotein a drug transporter in the brain microvessel isolated in rats our question is do the levels of p glycoprotein change at the blood brain barrier due to the drug treatments this one particular step will help us answer what we need to know the transfer of proteins is when we move the gel from electrophoresis onto a membrane we use filter paper to cover front and back of the metal plates this process is known as stacking then we use an electric current to run through the gel this pulls the proteins onto the membrane after the step is blocking and incubating then detection this process allows us to measure transporters that control drug delivery and also to find out if these transporters change due to disease or other drugs if a particular transporter is
