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well now that we have run our uh protein electrophoresis gel letamp;#39;s see how we can transfer uh the proteins to a membrane to do Western analysis so letamp;#39;s start by unboxing the ey blot dry blotting system letamp;#39;s see whatamp;#39;s in the box we have a manual on top here there we go so we have the device have a power cord this is a USB cable for uploading firmware and we have a roller for removing air bubbles so this is the I blot device Iamp;#39;m going to plug this in hereamp;#39;s the lid now letamp;#39;s take a look at the transfer Stacks so you have a choice of either pvdf or nitr cellulose each box comes with uh enough stacks for 10 transfers and they actually come in two sizes you can either get um a minisize one or you can get what I have here is the regular and this is large enough to transfer two mini gels at the same time so Inside the Box you have a bottom stack you have a top stack you have sponge and we also have a pack of filter papers weamp;#39;r