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true plot is Rocklandamp;#39;s premiere product line designed to produce publication quality Western blots from amino precipitation protocols in this video we will briefly show you how to use true blot from start to finish produce the pre-cleared lace8 add two micrograms of primary antibody and 50 microliters of pre-equilibrium Watt IP bead slurry to cold pre-cleared cell lysate incubate at 4 degrees Celsius for one hour or overnight for best results on a rocking platform or a rotator centrifuge the tube at 2500 G for 30 seconds at 4 degrees Celsius discard supernatant and watch the True Blood IP beads three to five times with 500 microliters of cold laces buffer centrifuge the tube at 2500 G for 30 seconds at 4 degrees Celsius between each wash discard the supernatant after each wash after the last wash add 50 microliters of 1x producing sds-page sample loading buffer with beta mercaptoethanol dithiothreitol or equivalent reducing agent to pellet vortex and heat to 90 to 100 degrees
