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a mic tag is a polypeptide protein tag derived from the Semite gene products that can be added to a protein using recombinant DNA technology it can be used for affinity chromatography then used to separate recombinant overexpressed protein from wild-type protein expressed by the host organism it can also be used in the isolation of protein complexes with multiple subunits a mic tag can be used in many different to says that require recognition by an antibody if there is no antibody against the studded protein adding a mic tag allows one to follow the protein with an antibody against the mic epitope examples are cellular localization studies by immunofluorescence or detection by Western blotting the peptide sequence of the mic tag is an eClass Edel si and Glu gln lisslow Yasir glue glue as blurr see where n stands for amino terminus and C stands for carboxy terminus the tag is approximately 1202 Daltonamp;#39;s in atomic mass and has 10 amino acids it can be fused to the C terminus and