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i remember i was really confused when i first saw these boxes um so these are western blot blotting boxes um and i was like why would you put why would you want a box that was black where you canamp;#39;t see things were in it whereas like if weamp;#39;re doing a classy stain we want a clear um box so you can actually see your protein being stained but when weamp;#39;re doing a western blot our secondary antibodies are typically light sensitive oftentimes these um antibodies are like conjugated or attached to a fluorophore so a fluorescent molecule and this allows us to shine light at one wavelength and thatamp;#39;ll give back light at a different wavelength that we can measure on a machine but if you shine light too much on those fluorophores um they kind of get like exhausted even if itamp;#39;s not the exact light that they want when you were talking about like white light we have all these different light ranges um and then thereamp;#39;s uv light thereamp;#39;s all sorts o
