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we will be performing a transfer as well as a western blot as stated in our video before we did perform an SDS page and within our SDS page our goal was to obtain a gel and the gel was to have protein bands that signified our desired egfp fusion protein so what we would do before we begin our transfers we will go ahead and cut all of these remaining Lanes in which we have used to insert our supernatant after we will go ahead and do that the purpose of doing so is because we will be placing our gel onto a Nitro cellulose membrane now this nitrocellulose membrane is known to bind two large amounts of proteins and the purpose of that is to obtain is to transfer the proteins from the gel onto the onto the membrane for our Western blot so weamp;#39;ll see here that this is how our apparatus should be looking light we do have our Tupperware in which in our scenario we do have to fill up with sodium bicarb and this is known as our transfer buffer so we will fill up our topper where in which
