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I second the use of secondary antibodies why savings and sensitivity basically by using a labeled secondary antibody that binds to a primary antibody that binds to the thing weamp;#39;re really interested in we can get a signal amplification so we get a stronger signal and we can save money because itamp;#39;s going to be a lot cheaper than trying to get your primary antibody the one that binds the thing of interest to be conjugated so let me explain we often use antibodies in the lab as probes so basically molecules that are going to allow us to detect the presence of specific other molecules these other molecules are often proteins such as in the case of a western blot or they can also be proteins or other molecules in blood such as from the presence of a virus so if a virus is in you and it makes a certain protein then a test called an Elisa for example can then use antibodies to detect for that specific protein in the blood and so these techniques rely on the fact that antibodies
