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in this video weamp;#39;ll be talking about Western blot this is a detailed explanation on Western blot Western blot is a frequently used technique in a molecular biology or biochemistry lab so what does this technique detect this technique can detect specific protein or your protein of interest in a mixture of protein or a patient sample so here is a mixture of protein and our question is whether this particular pink protein or our protein of interest is present within that mixture or not Western blot is a technique which can give us this answer and the way Western blot works is the antigen antibody interaction so here the protein of interest is detected by a primary antibody followed by a secondary antibody which is linked to some enzyme so this is why Western blot is also termed as immunoblot because it utilize the principle of antigen antibody interaction now letamp;#39;s talk about the Western block workflow in details so there there are broadly three sets of uh step First Step
