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hello friends welcome back to another video tutorial from shomos biology in this video lecture we want to talk about the western blotting technique so if you want to know what is western blot why western blot is used what are the steps of western blotting means the western blotting protocol then this video is just for you and watch this video to the end okay so the very first thing that i want to talk about of western blotting is that you need to have a basic and generic understanding of plotting techniques what is a blotting blotting is a technique which relies on the two different biological techniques one is the electrophoresis and the second one is the transfer a probe based binding method to detect specimen from a mixture of sample now in this case of western blotting the specimen that we want to detect is proteins for southern blotting the specimen that we want to detect is dna for northern blotting the specimen that we want to detect is rna so in western blotting we are detectin