Blot subject in AWW

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Aug 6th, 2022
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How to blot subject in AWW

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hello everyone in this video Iamp;#39;m going to speak about blotting techniques which are widely used techniques um in the biochemistry proteomics um and even in molecular biology of course in this video Iamp;#39;m going to speak about Western blotting there are also other blotting techniques so Western blotting is specifically used to detect proteins Southern blotting is used to detect DNA and Northern blotting to detect RNA in this video Iamp;#39;m going to speak about Western bloating and there is another video about southern blotting and Northern blotting so letamp;#39;s start speaking about a western blotting technique of course Western blotting is a technique used to detect proteins so itamp;#39;s used to detect a specific protein in a protein mixture letamp;#39;s say that I that I have a protein extract from my cell a prokaryotic cell or an eukaryotic cell whatever and I have a protein mixture and I am interested in the to detect a specific protein in this mixture what I

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In subject area: Nursing and Health Professions. Blotting refers to the transfer of macromolecules, such as proteins or nucleic acids, to a thin sheet of derivatized paper or adsorptive membrane matrix, allowing for easier and faster detection.
Western blotting is frequently used for the confirmatory medical diagnosis of infectious diseases such as Lyme disease, HIV infection, bovine spongiform encephalopathy (BSE), hepatitis C infection, syphilis, inflammatory muscle conditions such as myositis, and certain autoimmune disorders (e.g., paraneoplastic disease)
What is a western blot? The term blotting refers to the transfer of biological samples from a gel to a membrane and their subsequent detection on the surface of the membrane.
Western blotting is a laboratory technique used to detect a specific protein in a blood or tissue sample. The method involves using gel electrophoresis to separate the samples proteins.
The clinical significance of Western blotting is predominantly seen in its application for disease diagnosis. Diseases like cancer and autoimmune disorders often involve changes in protein expression levels. Western blotting can detect these changes, thereby assisting in disease diagnosis.
The Western blot test separates the blood proteins and detects the specific proteins (called HIV antibodies) that indicate an HIV infection.
Western blot protocol Stage 1 - Sample preparation. Stage 2 - Loading and running the gel. Stage 3 - Transferring from the gel to the membrane. Stage 4 - Checking the success of transfer (optional) Stage 5 - Blocking and antibody incubation. Stage 6 - Detection. Stage 7 - Membrane stripping (optional) Stage 8 - Data analysis.
In recent medical field, Western Blot has a wide range of applications in medical diagnosis, such as the application of medical diagnosis for HIV (Human Immunodeficiency Virus) infection, BSE (Bovine Spongiform Encephalopathy, also known as mad cow disease), FIV (Feline Immunodeficiency Virus), HBV (Hepatitis B Virus

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