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hello and welcome in this channel we break down complicated topics and easy to understand knowledgeable bits we added time stamps below for your convenience letamp;#39;s get started what problem do western blots solve for scientists well if youamp;#39;re working with an organism itamp;#39;s going to have a complex array of proteins and a western blot will give you information about whether the protein is present and in what amount itamp;#39;s a great way of separating out your protein of interest from all of the proteins within the cell system in the first step a buffer is added into a small test tube with the cells of your choice the buffer and some heat will start the process of denaturing the proteins from the complex 3d structures into their corresponding linear amino acids as it can be observed from a three-dimensional globular protein being denatured into a linear fashion is very important for the next step now that you added your cocktail of proteins to be able to accurately
