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western blotting western blotting is a technique using protein-specific antibodies to detect target proteins after gel electrophoresis proteins are transferred to a membrane and a blocking agent is used to cover places without proteins the membrane is then incubated with primary antibodies specific to the target protein secondary antibodies labeled with enzymes or fluorescent substances are then used to recognize the primary antibodies and produce signals this allows the identification of the target proteinamp;#39;s position and amount proteins are transferred to a hydrophobic pvdf membrane to increase interactions with antibodies due to the interaction with sds and gel electrophoresis the proteins carry negative charge and will migrate from the gel to the membrane under an electric current with the membrane facing the anode proteins will attach to the membrane via hydrophobic side chains completing the blotting process blocking agents should not have interactions with the tar
