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you might eventually run a Western blot that looks something like this image where the lanes are completely blank or there might be faint bands at the expected molecular weight if there are no bands at all on the blot the protein might not have transferred to the membrane which can be checked again with a pond so stain or maybe the secondary animal antibody is not compatible with the primary antibody itamp;#39;s possible that the sample does not express the protein of interest which can be checked using a positive control sample side by side with your unknown sample if the bands are very weak you can increase the amount of protein loaded and the concentration of the antibodies used you can change the blocking agent or block for a shorter period of time and you can increase the times of the antibody incubations if youamp;#39;re using a chemiluminescent detection I would also suggest exposing the film for a longer period of time to pick up any bands that might be on the block you could
