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okay so welcome to this next video in the playlist on experimental techniques in this video what weamp;#39;re going to talk about is the western blot okay so weamp;#39;re going to start off by discussing the motivation for the western blot what are we actually trying to do and then weamp;#39;ll discuss how you would actually do it in the theory that underlies it basically okay right so uh letamp;#39;s start off then with the uh motivation for the western bot so basically this is the motivation if you have a mixture containing many different proteins okay so letamp;#39;s say we have some um container here some beaker uh which contains some fluid here uh that contains a whole mixture of different proteins so letamp;#39;s say we have protein a here uh protein b here which will draw as a triangle and weamp;#39;ll have one more protein c so we wonamp;#39;t go any further than that but of course there could be a huge number of different proteins in this solution now basically we want