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[Music] bio rads v3 workflow provides researchers with more reliable quantitation and confidence in the western blotting process this is accomplished by first using tgx stain-free gels to separate and visualize protein samples after separation proteins are transferred to a membrane using the transblot turbo rapid transfer system which allows verification of the transfer quality and efficiency after verifying transfer the membrane is blocked and probed with primary and secondary antibodies for the target proteins finally target protein levels are normalized using the stain-free total protein measurement as a loading control the v3 workflow brings convenience and transparency to the western blotting process providing researchers with confidence at every step from separation to transfer and quantitation the main advantage of the v3 western workflow over traditional western blotting is that stainfree technology offers a practical convenient and reliable total protein loading control for
