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hello um as i go through biotechnology uh in a couple of uh videos uh this is going to focus on dna fingerprinting and its applications and then obviously uh the videos that are playing in the background they might go into the topics in a bit more detail this is an overview um before one can do a dna fingerprint one has to have enough dna but given that the sample size might have been small that could be a problem and so a a very useful technique is that of polymerase chain reaction or pcr and so lets say that you know you get blood from a patient or you find blood or a tissue sample at a crime scene uh or something and youd like enough uh dna uh to uh test uh where would um what would you do well obviously our cells replicate dna all the time um and so you could try to mimic that so that the um you have enough of a sample and so therefore youre going to have to go through a couple of steps uh repeatedly so the first step is your double-stranded molecule has to unwind now in your o