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this tutorial is a basic demonstration on how to perform total protein normalization using image lab software the first step is to acquire the images when acquiring the images it is important that you require the stain free blot image before adding any chemiluminescence substrate to the blot once the images are acquired we need to select the normalization channel this is done by selecting Blaine and bands in analysis toolbox then under the normalization channel drop down select add channel this brings up a window with the images available on the left side and the channels available on the right the chemi image has already been selected as our sample data and we will drag our total protein stain-free blot to the normalization channel then click okay next a window will automatically open up for band detection on our kimmieb lot for this example our band of interest has a strong signal so we will select low detection sensitivity and click OK image lab has automatically created all the la
